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Addgene

pcDNA3 GRK2 S670D
(Plasmid #45756)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45756 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GRK2 S670D
  • Alt name
    GRK2
  • Alt name
    beta adrenergic receptor kinase 1
  • Alt name
    ADRBK1
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    2300
  • Mutation
    S670D
  • Entrez Gene
    GRK2 (a.k.a. ADRBK1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The additional C619S mutation in the Addgene QC sequence does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 GRK2 S670D was a gift from Robert Lefkowitz (Addgene plasmid # 45756 ; http://n2t.net/addgene:45756 ; RRID:Addgene_45756)

  • For your References section:

    Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. Pitcher JA, Tesmer JJ, Freeman JL, Capel WD, Stone WC, Lefkowitz RJ. J Biol Chem. 1999 Dec 3. 274(49):34531-4. 10.1074/jbc.274.49.34531 PubMed 10574913
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