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Addgene

pcDNA3.0 FLAG TRPV4
(Plasmid #45751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TRPV4
  • Alt name
    transient receptor potential cation channel, subfamily V, member 4
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Trpv4 (a.k.a. Otrpc4, Vroac)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.0 FLAG TRPV4 was a gift from Robert Lefkowitz (Addgene plasmid # 45751 ; http://n2t.net/addgene:45751 ; RRID:Addgene_45751)

  • For your References section:

    Arresting a transient receptor potential (TRP) channel: beta-arrestin 1 mediates ubiquitination and functional down-regulation of TRPV4. Shukla AK, Kim J, Ahn S, Xiao K, Shenoy SK, Liedtke W, Lefkowitz RJ. J Biol Chem. 2010 Sep 24;285(39):30115-25. doi: 10.1074/jbc.M110.141549. Epub 2010 Jul 22. 10.1074/jbc.M110.141549 PubMed 20650893
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