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Addgene

pET29b-Ava3858-3857
(Plasmid #45744)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45744 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5238
  • Total vector size (bp) 7485
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ava3858-3857
  • Alt name
    DHQS homolog
  • Alt name
    O-methyltransferase
  • Species
    Anabaena variabilis
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-Term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-Ava3858-3857 was a gift from Emily Balskus & Christopher T Walsh (Addgene plasmid # 45744 ; http://n2t.net/addgene:45744 ; RRID:Addgene_45744)

  • For your References section:

    The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Balskus EP, Walsh CT. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2. 10.1126/science.1193637 PubMed 20813918
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