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pET28b-Ava3855
(Plasmid #45743)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45743 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5293
  • Total vector size (bp) 7960
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ava3855
  • Alt name
    NRPS-like enzyme
  • Alt name
    amino acid adenylation protein
  • Species
    Anabaena variabilis
  • Insert Size (bp)
    2667
  • GenBank ID
    YP_324355.1
  • Entrez Gene
    Ava_3855 (a.k.a. Ava_3855)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-Term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-Ava3855 was a gift from Emily Balskus & Christopher T Walsh (Addgene plasmid # 45743 ; http://n2t.net/addgene:45743 ; RRID:Addgene_45743)

  • For your References section:

    The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Balskus EP, Walsh CT. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2. 10.1126/science.1193637 PubMed 20813918
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