pET29b-NpR5599
(Plasmid
#45740)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET29b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5238
- Total vector size (bp) 6069
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNpR5599
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Alt nameO-methyltransferase
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SpeciesNostoc punctiforme
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Insert Size (bp)831
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GenBank IDACC83904 YP_001868847.1
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Entrez GeneNpun_R5599 (a.k.a. Npun_R5599)
- Promoter T7
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-Term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29b-NpR5599 was a gift from Emily Balskus & Christopher T Walsh (Addgene plasmid # 45740 ; http://n2t.net/addgene:45740 ; RRID:Addgene_45740) -
For your References section:
The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Balskus EP, Walsh CT. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2. 10.1126/science.1193637 PubMed 20813918
Map uploaded by the depositor.
