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pBp-FGFR2c-E471Q
(Plasmid #45704)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe puro Gateway
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7900
  • Modifications to backbone
    Gateway cloning sequences flank region with gene insert for use with the Gateway system reagents.
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FGFR2 IIIc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Mutation
    E471Q
  • Entrez Gene
    FGFR2 (a.k.a. BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer Gateway reagents
  • 3′ sequencing primer Gateway reagents
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBp-FGFR2c-E471Q was a gift from Matthew Meyerson (Addgene plasmid # 45704 ; http://n2t.net/addgene:45704 ; RRID:Addgene_45704)

  • For your References section:

    Inhibitor-sensitive FGFR2 and FGFR3 mutations in lung squamous cell carcinoma. Liao RG, Jung J, Tchaicha JH, Wilkerson MD, Sivachenko A, Beauchamp EM, Liu Q, Pugh TJ, Pedamallu CS, Hayes DN, Gray NS, Getz G, Wong KK, Haddad RI, Meyerson M, Hammerman PS. Cancer Res. 2013 Jun 20. 10.1158/0008-5472.CAN-12-3950 PubMed 23786770
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