pCRII-Topo Nectin-3 in situ probe
(Plasmid #45633)

• Depositing Lab: Jeffrey Macklis
• Publication: Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54. doi: 10.1523/JNEUROSCI.6108-08.2009.

Backbone

• Vector backbone: pCRII-Topo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4400
• Vector type: in situ

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Nectin-3 in situ probe
• Alt name: Nectin-3
• Alt name: Pvrl3
• Alt name: poliovirus receptor-related 3
• Species: M. musculus (mouse)
• Insert Size (bp): 415
• Mutation: fragment contains bp# 1626-2039 of NM_021495
• GenBank ID: NM_021495
• Entrez Gene: Nectin3 (a.k.a. 2610301B19Rik, 3000002N23Rik, 4921513D19Rik, AA407785, AU016832, AW538082, CD113, P, Pvrl3, necti)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13 Rev, Sp6
• 3′ sequencing primer: T7, M13 Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Nectin-3 fragment was amplified by RT-PCR using the following primer pair:
AAACAACCTGATCCGCAAAG
CAGTGAAAACTGTAAAGCAGCTC

For in vitro transcription, use the BamHI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.

Please note that Addgene's sequencing results match bp# 1626-2039 of NM_021495, not bp# 1624-2036 as indicated on plasmid map. The plasmid functions as described in the associated publication.