pSABAD544
(Plasmid
#45617)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 4597
- Total vector size (bp) 5704
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFPN-NpuDnaE_AA-GFP_C
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Alt nameinactive NpuDnaE inserted in GFP chromophore
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SpeciesNostoc punctiforme
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Insert Size (bp)1107
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MutationInactivating mutations in NpuDnaE C-terminus, stemmer GFP with superfolder mutations S30R and Y39N, and SYG->CYG in chromophore
- Promoter pBAD
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSABAD544 was a gift from Hideo Iwai (Addgene plasmid # 45617 ; http://n2t.net/addgene:45617 ; RRID:Addgene_45617) -
For your References section:
Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115
Map uploaded by the depositor.
