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Addgene

pSABAD505
(Plasmid #45615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 4597
  • Total vector size (bp) 5704
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFPN-NpuDnaE_intein-GFP_C
  • Alt name
    NpuDnaE inserted in GFP chromophore
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    1107
  • Mutation
    stemmer GFP with superfolder mutations S30R and Y39N, and SYG->CYG in chromophore
  • Promoter pBAD
  • Tags / Fusion Proteins
    • GFP_N (N terminal on insert)
    • GFP_C (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBAD Forward
  • 3′ sequencing primer pBAD Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSABAD505 was a gift from Hideo Iwai (Addgene plasmid # 45615 ; http://n2t.net/addgene:45615 ; RRID:Addgene_45615)

  • For your References section:

    Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115
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