pJORSF19
(Plasmid
#45614)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSF-1
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Backbone manufacturerMerckMillipore
- Backbone size w/o insert (bp) 3559
- Total vector size (bp) 5152
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePhoRadA precursor
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Alt namePhoRadA including exteins and intein
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Speciespyrococcus horikoshii
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Insert Size (bp)1593
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJORSF19 was a gift from Hideo Iwai (Addgene plasmid # 45614 ; http://n2t.net/addgene:45614 ; RRID:Addgene_45614) -
For your References section:
Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115