pSADuet760
(Plasmid
#45613)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSFDuet-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3564
- Total vector size (bp) 4479
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Modifications to backbonedeletion of MCS-2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMjaTFIIB mini intein
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Alt nameminimized NpuDnaE intein
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SpeciesMethanococcus jannaschii
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Insert Size (bp)915
- Promoter T7
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Tags
/ Fusion Proteins
- H6-GB1 (N terminal on insert)
- GB1 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSADuet760 was a gift from Hideo Iwai (Addgene plasmid # 45613 ; http://n2t.net/addgene:45613 ; RRID:Addgene_45613) -
For your References section:
Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115
Map uploaded by the depositor.
