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Addgene

pHYDuet216A
(Plasmid #45611)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45611 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3564
  • Total vector size (bp) 4380
  • Modifications to backbone
    deletion of MCS-2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaE delta variant
  • Alt name
    minimized NpuDnaE intein
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    816
  • Mutation
    deleted T76, V97, D98, L100 from NpuDnaE intein
  • Promoter T7
  • Tags / Fusion Proteins
    • H6-GB1 (N terminal on insert)
    • GB1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYDuet216A was a gift from Hideo Iwai (Addgene plasmid # 45611 ; http://n2t.net/addgene:45611 ; RRID:Addgene_45611)

  • For your References section:

    Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115
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