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pCI-neo-alpha4-SEP
(Plasmid #45607)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45607 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 8112
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nAChRAlpha4-SEP
  • Alt name
    Alpha4-SEP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2640
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • SEP- Tag (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Alpha4 WT was originally received from Dr. J Stitzel, University of Colorado, Boulder

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PI: Henry Lester

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-alpha4-SEP was a gift from Henry Lester (Addgene plasmid # 45607 ; http://n2t.net/addgene:45607 ; RRID:Addgene_45607)

  • For your References section:

    Trafficking of {alpha}4* Nicotinic Receptors Revealed by Superecliptic Phluorin: EFFECTS OF A {beta}4 AMYOTROPHIC LATERAL SCLEROSIS-ASSOCIATED MUTATION AND CHRONIC EXPOSURE TO NICOTINE. Richards CI, Srinivasan R, Xiao C, Mackey ED, Miwa JM, Lester HA. J Biol Chem. 2011 Sep 9;286(36):31241-9. Epub 2011 Jul 18. 10.1074/jbc.M111.256024 PubMed 21768117
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