pMMDuet19
(Plasmid #45594)

• Depositing Lab: Hideo Iwai
• Publication: Ellila et al FEBS Lett. 2011 Oct 10.

Backbone

• Vector backbone: pRSFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3564
• Total vector size (bp): 4419
• Modifications to backbone: removed the second MCS of pRSFDuet-1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NpuDnaB mini intein
• Alt name: cis-splicing intein with H6-GB1 and GB1 as N- and C-exteins
• Species: Nostoc punctiforme
• Insert Size (bp): 855
• Mutation: removal of endonuclease domain
• Promoter: T7
• Tags / Fusion Proteins:
  • H6-GB1 (N terminal on insert)
  • GB1 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry