pEGFP-TRIM28
(Plasmid #45568)

• Depositing Lab: Fanxiu Zhu
• Publication: Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. doi: 10.4049/jimmunol.1101704. Epub 2011 Sep 21.

Backbone

• Vector backbone: pEGFP-C
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRIM28
• Alt name: tripartite motif containing 28
• Alt name: KAP1
• Alt name: TIF1β
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Mutation: G34S mutation in EGFP
• GenBank ID: NP_005753.1
• Entrez Gene: TRIM28 (a.k.a. KAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: EGFP-C; CMV-F
• 3′ sequencing primer: SV40-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Full-length human TRIM28 was purchased from Origene and subcloned into vector pKH3 to generate pKH3-Trim28 (Addgene plasmid #45569). A full length TRIM28 DNA fragment was then generated from pKH3-TRIM28 by PCR and cloned into pEGFP.

The depositing laboratory indicated that the G34S mutation in EGFP does not affect the function of the EGFP-TRIM28 fusion protein.