pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
(Plasmid #45565)

• Purpose: Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker
• Depositing Lab: Andrew Scharenberg
• Publication: Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Backbone

• Vector backbone: pExodus
• Backbone size w/o insert (bp): 3724
• Total vector size (bp): 6112
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: I-Sce I Nuclease
• Species: H. sapiens (human)
• Insert Size (bp): 711
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: BFP
• Species: H. sapiens (human)
• Insert Size (bp): 762

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry