JAF99.4
(Plasmid
#45560)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEGFP-C2
-
Backbone manufacturerClontech
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePkhd1
-
SpeciesM. musculus (mouse)
-
MutationContains only the cytoplasmic tail (last 193 amino acids)
-
GenBank IDNM_153179.2
-
Entrez GenePkhd1 (a.k.a. FPC, Tigm1)
- Promoter CMV
-
Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
JAF99.4 was a gift from Gregory Pazour (Addgene plasmid # 45560 ; http://n2t.net/addgene:45560 ; RRID:Addgene_45560) -
For your References section:
The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence. Follit JA, Li L, Vucica Y, Pazour GJ. J Cell Biol. 2010 Jan 11;188(1):21-8. doi: 10.1083/jcb.200910096. Epub 2010 Jan 4. 10.1083/jcb.200910096 PubMed 20048263