BD46.32
(Plasmid
#45559)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45559 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSstr3
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SpeciesM. musculus (mouse)
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Entrez GeneSstr3 (a.k.a. Smstr-3, Smstr3, sst3)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BD46.32 was a gift from Gregory Pazour (Addgene plasmid # 45559 ; http://n2t.net/addgene:45559 ; RRID:Addgene_45559) -
For your References section:
Targeting proteins to the ciliary membrane. Pazour GJ, Bloodgood RA. Curr Top Dev Biol. 2008;85:115-49. doi: 10.1016/S0070-2153(08)00805-3. 10.1016/S0070-2153(08)00805-3 PubMed 19147004