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Addgene

BD46.32
(Plasmid #45559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sstr3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Sstr3 (a.k.a. Smstr-3, Smstr3, sst3)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BD46.32 was a gift from Gregory Pazour (Addgene plasmid # 45559 ; http://n2t.net/addgene:45559 ; RRID:Addgene_45559)

  • For your References section:

    Targeting proteins to the ciliary membrane. Pazour GJ, Bloodgood RA. Curr Top Dev Biol. 2008;85:115-49. doi: 10.1016/S0070-2153(08)00805-3. 10.1016/S0070-2153(08)00805-3 PubMed 19147004
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