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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45525 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePKA regulatory subunit RI alpha tagged by mEGFP
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-mouse PKA-RIalpha-mEGFP was a gift from Haining Zhong (Addgene plasmid # 45525 ; http://n2t.net/addgene:45525 ; RRID:Addgene_45525) -
For your References section:
Subcellular dynamics of type II PKA in neurons. Zhong H, Sia GM, Sato TR, Gray NW, Mao T, Khuchua Z, Huganir RL, Svoboda K. Neuron. 2009 May 14;62(3):363-74. doi: 10.1016/j.neuron.2009.03.013. 10.1016/j.neuron.2009.03.013 PubMed 19447092