pcDNA3.1-Flag-PGC-1alpha2
(Plasmid #45502)

• Depositing Labs: Jorge Ruas, Bruce Spiegelman
• Publication: Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10.1016/j.cell.2012.10.050.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5424
• Total vector size (bp): 6564
• Modifications to backbone: Modified MCS to include a Flag-tag cDNA sequence between BamHI and NotI sites.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PGC-1alpha Isoform2
• Alt name: PGC-1alpha2
• Alt name: PGC-1a2
• Species: M. musculus (mouse)
• Insert Size (bp): 1140
• GenBank ID: JX866946
• Entrez Gene: Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry