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Addgene

pcDNA3-DKAR-Nuc
(Plasmid #45499)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45499 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SURE
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DKAR-Nuc
  • Alt name
    Protein Kinase D activity reporter
  • Species
    Synthetic
  • Insert Size (bp)
    2013
  • Promoter CMV
  • Tags / Fusion Proteins
    • CFP (N terminal on insert)
    • YFP (C terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (unknown if destroyed)
  • 3′ cloning site Xba I (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FRET-based PKD activation reporter (DKAR). Contains a PKD-specific substrate and phosphoamino acid-binding domain linking CFP and YFP. Upon phosphorylation by PKD, FRET is reduced.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-DKAR-Nuc was a gift from Alexandra Newton (Addgene plasmid # 45499 ; http://n2t.net/addgene:45499 ; RRID:Addgene_45499)

  • For your References section:

    Spatiotemporally distinct protein kinase D activation in adult cardiomyocytes in response to phenylephrine and endothelin. Bossuyt J, Chang CW, Helmstadter K, Kunkel MT, Newton AC, Campbell KS, Martin JL, Bossuyt S, Robia SL, Bers DM. J Biol Chem. 2011 Sep 23;286(38):33390-400. doi: 10.1074/jbc.M111.246447. Epub 2011 Jul 27. 10.1074/jbc.M111.246447 PubMed 21795686
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