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Addgene

pLV-tetO YY1
(Plasmid #45498)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45498 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLV-tetO (PMID: 18371448)
  • Backbone manufacturer
    Konrad Hochedlinger lab (MGH, Boston, MA)
  • Modifications to backbone
    Backbone is modified from FUGW (Addgene plasmid 14883) by replacing the hUbC_promoter-EGFP-containing PacI-EcoRI fragment with tetO-CMV promoter-containing XhoI-EcoRI fragment from pTet-Splice
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YY1
  • Alt name
    YY1 transcription factor
  • Alt name
    Yin Yang 1
  • Alt name
    NF-E1
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    YY1 (a.k.a. DELTA, GADEVS, INO80S, NF-E1, UCRBP, YIN-YANG-1)
  • Promoter TetO-CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    YY1 cDNA from Dr. Edward Seto, Moffitt Cancer Center, Florida. pLV-tetO plasmid backbone from Konrad Hochedlinger, MGH, Boston, MA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-tetO YY1 was a gift from Sean Wu (Addgene plasmid # 45498)

  • For your References section:

    Essential and unexpected role of Yin Yang 1 to promote mesodermal cardiac differentiation. Gregoire S, Karra R, Passer D, Deutsch MA, Krane M, Feistritzer R, Sturzu A, Domian I, Saga Y, Wu SM. Circ Res. 2013 Mar 15;112(6):900-10. doi: 10.1161/CIRCRESAHA.113.259259. Epub 2013 Jan 10. 10.1161/CIRCRESAHA.113.259259 PubMed 23307821
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