Skip to main content
Addgene

pNkx-GFP
(Plasmid #45497)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45497 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4151
  • Total vector size (bp) 6766
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    2.5kb mouse Nkx2.5 enhancer and promoter fragment
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • GenBank ID
    NC_000083.6
  • Entrez Gene
    Nkx2-5 (a.k.a. Csx, Nkx-2.5, Nkx2.5, tinman)
  • Promoter 2.5kb Nkx2.5 enhancer and promoter fragment
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer EGFP-N; SV40-pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    2.1 kb murine Nkx2.5 enhancer fragment from Dr. Eric Olson, UT Southwestern, Dallas, TX, by MTA with Children's Hospital, Boston, MA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternate plasmid name: Nkx2.5 cardiac enhancer-base promoter-eGFP

The full plasmid sequence is approximate and may not be entirely accurate.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNkx-GFP was a gift from Sean Wu (Addgene plasmid # 45497 ; http://n2t.net/addgene:45497 ; RRID:Addgene_45497)

  • For your References section:

    Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart. Wu SM, Fujiwara Y, Cibulsky SM, Clapham DE, Lien CL, Schultheiss TM, Orkin SH. Cell. 2006 Dec 15;127(6):1137-50. Epub 2006 Nov 22. 10.1016/j.cell.2006.10.028 PubMed 17123591
Related items:
Commonly requested with: