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Addgene

Di-V_7_R3x1_7
(Plasmid #45488)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b(+)
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 5545
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Growth instructions
    For protein expression, BL21 cell is recommended
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Di-V_7_R3x1_7
  • Alt name
    R3x3_Npart_7
  • Species
    Synthetic
  • Insert Size (bp)
    315
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GenScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Di-V_7:
mgSKIIVIISSDDTTLEELARKIKDEGLEVYILLKDKDEKRLEEKIQKLKSQGFEVRKVKDDDDIDKWIDKIKKERPQLEVRKVTDEDQAKQILEDLKKKGslehhhhhh

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Di-V_7_R3x1_7 was a gift from David Baker (Addgene plasmid # 45488 ; http://n2t.net/addgene:45488 ; RRID:Addgene_45488)

  • For your References section:

    Principles for designing ideal protein structures. Koga N, Tatsumi-Koga R, Liu G, Xiao R, Acton TB, Montelione GT, Baker D. Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600. 10.1038/nature11600 PubMed 23135467
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