pTLPflaG-small
(Plasmid
#45476)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTLP6
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 5600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsUse Chloramphenicol at 10ug/mL.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameflaA-GFP
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Alt nameMB360
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SphI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer GFPfusionREV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The ~1kb BamHI/SphI flaAGFP fragment from pflaG into the ~4.6kb BamHI/SphI fragment of the ColE1 replicon pTLP6.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTLPflaG-small was a gift from Michele Swanson (Addgene plasmid # 45476 ; http://n2t.net/addgene:45476 ; RRID:Addgene_45476) -
For your References section:
Co-ordination of legionella pneumophila virulence with entry into stationary phase by ppGpp. Hammer BK, Swanson MS. Mol Microbiol. 1999 Aug;33(4):721-31. 10.1046/j.1365-2958.1999.01519.x PubMed 10447882