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Addgene

pMMBGent
(Plasmid #45475)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45475 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMB67EH
  • Backbone manufacturer
    Bagdasarian Lab
  • Modifications to backbone
    The Gentamycin cassette was Klenow treated and ligated into the DraI site in pMMB67EH. This inactivates the amp cassette.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gentamycin
  • Alt name
    MB356

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer M13-Rev
  • 3′ sequencing primer pBAD-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a vector control for IPTG-indicible E. coli relA gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMBGent was a gift from Michele Swanson (Addgene plasmid # 45475 ; http://n2t.net/addgene:45475 ; RRID:Addgene_45475)

  • For your References section:

    Co-ordination of legionella pneumophila virulence with entry into stationary phase by ppGpp. Hammer BK, Swanson MS. Mol Microbiol. 1999 Aug;33(4):721-31. 10.1046/j.1365-2958.1999.01519.x PubMed 10447882
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