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Addgene

HB101 endA::frt lambda pir
(Bacterial strain #45472)

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Full plasmid sequence is not available for this item.

No maps are available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 45472 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    HB101 lambda pir
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    endA::frt lambda pir
  • Alt name
    MB740
  • Mutation
    major endonuclease endA deleted

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use lambda pir strain for R6K ori replication. endA was deleted for cleaner and higher yield plasmid preps.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HB101 endA::frt lambda pir was a gift from Michele Swanson (Addgene plasmid # 45472)

  • For your References section:

    Oligonucleotides stimulate genomic alterations of Legionella pneumophila. Bryan A, Swanson MS. Mol Microbiol. 2011 Apr;80(1):231-47. doi: 10.1111/j.1365-2958.2011.07573.x. Epub 2011 Feb 24. 10.1111/j.1365-2958.2011.07573.x PubMed 21306445
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