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Addgene

pBSFlp
(Plasmid #45470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45470 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMB206
  • Backbone manufacturer
    Bagdasarian Lab
  • Total vector size (bp) 7894
  • Modifications to backbone
    pMMB origin and amp cassette replaced with pBluescript ori and amp cassette
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    This construct is also gentamycin resistant.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flp recombinase
  • Alt name
    MB791
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13-Rev
  • 3′ sequencing primer M13-F20
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is unstable in Legionella and may be slightly unstable in E. coli.
Please note that the sacB confers sucrose sensitivity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSFlp was a gift from Michele Swanson (Addgene plasmid # 45470 ; http://n2t.net/addgene:45470 ; RRID:Addgene_45470)

  • For your References section:

    Efficient generation of unmarked deletions in Legionella pneumophila. Bryan A, Harada K, Swanson MS. Appl Environ Microbiol. 2011 Apr;77(7):2545-8. doi: 10.1128/AEM.02904-10. Epub 2011 Feb 4. 10.1128/AEM.02904-10 PubMed 21296953
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