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pR6Kfrt-gent-frt
(Plasmid #45469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45469 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pR6K
  • Backbone manufacturer
    Datensko and Wanner
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5a lambda pir
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    frt-gent-frt
  • Alt name
    MB838

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer R6K-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

frt-gent-frt cassette was cut out of pGEM-frt-gent-frt with EcoRI and ligated into EcoRI digested pR6K. Please note that the R6K ori may be in opposite orientation relative to cassette and annotation since it was not directionally cloned.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pR6Kfrt-gent-frt was a gift from Michele Swanson (Addgene plasmid # 45469)

  • For your References section:

    Efficient generation of unmarked deletions in Legionella pneumophila. Bryan A, Harada K, Swanson MS. Appl Environ Microbiol. 2011 Apr;77(7):2545-8. doi: 10.1128/AEM.02904-10. Epub 2011 Feb 4. 10.1128/AEM.02904-10 PubMed 21296953
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