pNLS-iRFP720
(Plasmid #45467)

• Depositing Lab: Vladislav Verkhusha
• Publication: Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.

Backbone

• Vector backbone: pN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4055
• Total vector size (bp): 5006
• Modifications to backbone: nuclear localization signal
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iRFP720
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 951
• Mutation: T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193K, F198Y, D202F, V254N, Y258F, A283V, K288T, N290Y relative to RpBphP2
• GenBank ID: KC991145
• Promoter: CMV
• Tag / Fusion Protein:
  • nuclear localization signal (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry