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Addgene

pMito-iRFP720
(Plasmid #45464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45464 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4038
  • Total vector size (bp) 4989
  • Modifications to backbone
    human cytochrome C oxidase subunit VIII
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iRFP720
  • Species
    Rhodopseudomonas palustris
  • Insert Size (bp)
    951
  • Mutation
    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193K, F198Y, D202F, V254N, Y258F, A283V, K288T, N290Y relative to RpBphP2
  • GenBank ID
    KC991145
  • Promoter CMV
  • Tag / Fusion Protein
    • human cytochrome C oxidase subunit VIII (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMito-iRFP720 was a gift from Vladislav Verkhusha (Addgene plasmid # 45464 ; http://n2t.net/addgene:45464 ; RRID:Addgene_45464)

  • For your References section:

    Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova DM, Verkhusha VV. Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521. 10.1038/nmeth.2521 PubMed 23770755
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