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Addgene

pBbA5k-EPL69
(Plasmid #45428)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbA5k
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH1 with AcrAB knocked out
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Gmet_2518
  • Species
    Synthetic
  • Insert Size (bp)
    5642
  • Promoter LacUV5
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CAA AAG ATC TTT TAA GAA GGA GAT ATA CAT ATG AAC GTA TCA AAA ATA TGC
  • 3′ sequencing primer CAG TGG TGA TGG TGA TGA TGG AGA CTT CCC TCC CCC AGC ACC C
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ATCC 53774D-5

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5k-EPL69 was a gift from Aindrila Mukhopadhyay (Addgene plasmid # 45428 ; http://n2t.net/addgene:45428 ; RRID:Addgene_45428)

  • For your References section:

    Engineering microbial biofuel tolerance and export using efflux pumps. Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi MZ, Mukhopadhyay A. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. 10.1038/msb.2011.21 PubMed 21556065
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