pBbA5k-EPL14
(Plasmid #45405)

• Depositing Lab: Aindrila Mukhopadhyay
• Publication: Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.

Backbone

• Vector backbone: pBbA5k
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH1 with AcrAB knocked out
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mexF, PP_3426
• Species: Synthetic
• Insert Size (bp): 5846
• Promoter: LacUV5
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CAA AAG ATC TTT TAA GAA GGA GAT ATA CAT ATG GAA CAA TCA CTC AAA CCT TTG C
• 3′ sequencing primer: CAG TGG TGA TGG TGA TGA TGG TGC GCG CTG GCC ACC GTC TCC G

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.