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Addgene

pBEST-p15A-PLtetO1-tetR-T500
(Plasmid #45391)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45391 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBEST-Luc
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4486
  • Total vector size (bp) 4486
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tet repressor
  • Alt name
    tetR
  • Species
    e. coli
  • Insert Size (bp)
    623
  • GenBank ID
    BAG71042.1
  • Promoter PL-tetO1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site sphI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEST-p15A-PLtetO1-tetR-T500 was a gift from Vincent Noireaux (Addgene plasmid # 45391 ; http://n2t.net/addgene:45391 ; RRID:Addgene_45391)

  • For your References section:

    Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. Shin J, Noireaux V. J Biol Eng. 2010 Jun 24;4:8. 10.1186/1754-1611-4-8 PubMed 20576148
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