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Addgene

pBluescript SK-rPAI-153
(Plasmid #45389)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 6100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Plasminogen activator inhibitor type 1
  • Alt name
    PAI-1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3100
  • GenBank ID
    M24067
  • Entrez Gene
    Serpine1 (a.k.a. PAI1A, Pai1, Pai1aa, Planh, RATPAI1A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript SK-rPAI-153 was a gift from Thomas Gelehrter (Addgene plasmid # 45389 ; http://n2t.net/addgene:45389 ; RRID:Addgene_45389)

  • For your References section:

    Cloning and sequencing of cDNA for the rat plasminogen activator inhibitor-1. Zeheb R, Gelehrter TD. Gene. 1988 Dec 20;73(2):459-68. 10.1016/0378-1119(88)90510-0 PubMed 3149611
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