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pMAL-BRT17
(Plasmid #45358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-c4e
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6648
  • Total vector size (bp) 7083
  • Modifications to backbone
    Knocked out native enterokinase cleavage site (2677-2691) from DDDDK to DDGEQ
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    17 Repeat Beta Roll Tag
  • Alt name
    BRT17
  • Species
    Synthetic
  • Insert Size (bp)
    462
  • Promoter Ptac
  • Tag / Fusion Protein
    • Maltose Binding Protein (MBP) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CGT CAG ACT GTC GAT GAA
  • 3′ sequencing primer CGC CAG GGT TTT CCC AGT CAC GAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL-BRT17 was a gift from Scott Banta (Addgene plasmid # 45358 ; http://n2t.net/addgene:45358 ; RRID:Addgene_45358)

  • For your References section:

    A designed, phase changing RTX-based peptide for efficient bioseparations. Shur O, Dooley K, Blenner M, Baltimore M, Banta S. Biotechniques. 2013 Apr;54(4):197-206. doi: 10.2144/000114010. 10.2144/000114010 PubMed 23581466
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