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Addgene

UPR pBAM Ura3
(Plasmid #45355)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAM
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DHFR Ub Mek Ura3
  • Alt name
    UPR pBAM Ura3 DHFR Ub Met ek Ura3
  • Promoter pCUP1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer yCUP1pro-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UPR pBAM Ura3 was a gift from Alexander Varshavsky (Addgene plasmid # 45355 ; http://n2t.net/addgene:45355 ; RRID:Addgene_45355)

  • For your References section:

    Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway. Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A. J Biol Chem. 2008 Aug 29. 283(35):24011-28. 10.1074/jbc.M802583200 PubMed 18566452
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