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Addgene

pR208IM1
(Plasmid #45351)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45351 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRB208
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBR1 mutant
  • Alt name
    empty ADH 2 micron LEU UBR1 type 1 mutant IM1
  • Mutation
    D176E
  • Promoter ADH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer yADH1-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a complementation plasmid where the SpeI + BglII fragment of the original pRBUBR1IM1 plasmid was ligated into pRB208 digested with SpeI + BglII.

Plasmid overexpresses a mutant Ubr1pha that is able to target type II but not type I N-end rule substrates for degradation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pR208IM1 was a gift from Alexander Varshavsky (Addgene plasmid # 45351 ; http://n2t.net/addgene:45351 ; RRID:Addgene_45351)

  • For your References section:

    Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway. Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A. J Biol Chem. 2008 Aug 29. 283(35):24011-28. 10.1074/jbc.M802583200 PubMed 18566452
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