pCU-LYS21-GFP
(Plasmid
#45332)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45332 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGRB2.3
- Total vector size (bp) 7140
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Modifications to backbonePDC1 promoter C. glabrata LYS21 promoter (cut SacI/SpeI) was cloned into pGRB2.3 backbone (cut SacI/XbaI), replacing the PGK2 promoter originally found in pGRB2.3
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Vector typeC. glabrata expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameyeGFP
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Alt nameGFP
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SpeciesSynthetic
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Insert Size (bp)717
- Promoter LYS21
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCU-LYS21-GFP was a gift from Brendan Cormack (Addgene plasmid # 45332 ; http://n2t.net/addgene:45332 ; RRID:Addgene_45332) -
For your References section:
Expression plasmids for use in Candida glabrata. Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, Cormack BP. G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908. 10.1534/g3.113.006908 PubMed 23934995
