pCN-ACO2-GFP
(Plasmid #45330)

• Depositing Lab: Brendan Cormack
• Publication: Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.

Backbone

• Vector backbone: pBM16
• Total vector size (bp): 7694
• Modifications to backbone: SacI/Acc65I digest used to move ACO2pr-GFP-terminator fragment from pCU-ACO2-GFP to the pBM16 backbone. Note the ACO2 promoter naturally contains an Acc65I site, so a complete SacI/partial Acc65I digest was used to mobilize the fragment.
• Vector type: C. glabrata expression
• Selectable markers: nourseothricin (NAT)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: yeGFP
• Alt name: GFP
• Species: Synthetic
• Insert Size (bp): 717
• Promoter: ACO2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.