pCU-PDC1
(Plasmid
#45323)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGRB2.1
- Backbone size (bp) 6254
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Modifications to backboneC. glabrata PDC1 promoter inserted into pGRB2.1 backbone using SacI/XbaI restricton digest
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Vector typeC. glabrata expression
- Promoter PDC1
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCU-PDC1 was a gift from Brendan Cormack (Addgene plasmid # 45323 ; http://n2t.net/addgene:45323 ; RRID:Addgene_45323) -
For your References section:
Expression plasmids for use in Candida glabrata. Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, Cormack BP. G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908. 10.1534/g3.113.006908 PubMed 23934995