pLEX_970_puro_DEST_YFP
(Plasmid
#45295)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLEX_970
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYFP
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pLEX-fwd
- 3′ sequencing primer pLEX-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe YFP gene cloned from the pEntry-YFP vector in Addgene.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The YFP open reading frame was PCR amplified with BP flanked primers and adding an open ending to the YFP gene. The PCR product was ligated into pDONR223 with a Gateway BP reaction and subsequently cloned into pLEX970 with a Gateway LR reaction.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX_970_puro_DEST_YFP was a gift from William Hahn (Addgene plasmid # 45295 ; http://n2t.net/addgene:45295 ; RRID:Addgene_45295) -
For your References section:
Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer drivers. Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P, Mesirov JP, Beroukhim R, Hahn WC. Cancer Discov. 2013 Jun 13. [Epub ahead of print] 10.1158/2159-8290.CD-12-0592 PubMed 23764425