pBabe_puro_DEST_Flag_TNIK
(Plasmid #45276)

• Depositing Lab: William Hahn
• Publication: Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead of print]

Backbone

• Vector backbone: pBabe-puro-flag-DEST
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TNIK
• Alt name: TNIK
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: BC055427
• Entrez Gene: TNIK (a.k.a. MRT54)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pBabe 5'
• 3′ sequencing primer: pBabe 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Open Biosystems MHS1010-9203902
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The TNIK open reading frame was PCR amplified with BP flanked primers from the vector acquired from Open Biosystems and ligated into a pDONR223 vector in a BP Gateway ligation reaction. The TNIK open reading frame was subsequently ligated into the pBabe_puro_DEST_flag backbone in a Gateway LR ligation reaction.