pBabe_puro_DEST_Flag_SOX2
(Plasmid #45270)

• Depositing Lab: William Hahn
• Publication: Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead of print]

Backbone

• Vector backbone: pBabe-puro-flag-DEST
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCOPS3
• Species: H. sapiens (human)
• Insert Size (bp): 954
• Entrez Gene: SOX2 (a.k.a. ANOP3, MCOPS3)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pBabe 5'
• 3′ sequencing primer: pBabe 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: THE CCSB HUMAN ORFEOME COLLECTION

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry