pBabe_puro_DEST_Flag_EIF5A2
(Plasmid #45266)

• Depositing Lab: William Hahn
• Publication: Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead of print]

Backbone

• Vector backbone: pBabe-puro-flag-DEST
• Total vector size (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EIF5A2
• Species: H. sapiens (human)
• Entrez Gene: EIF5A2 (a.k.a. EIF-5A2, eIF5AII)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pBabe5'
• 3′ sequencing primer: pBabe3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: THE CCSB HUMAN ORFEOME COLLECTION
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry