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Addgene

Cenp-B DBD INCENP GFP
(Plasmid #45237)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45237 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cenp-B-DBD, INCENP-Δcen, GFP
  • Alt name
    pXW031
  • Species
    H. sapiens (human)
  • Mutation
    CEN box deleted from INCENP (aa1-46)
  • Tags / Fusion Proteins
    • GFP (C terminal on insert)
    • DNA binding domain of CENP-B

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cenp-B DBD INCENP GFP was a gift from Michael Lampson (Addgene plasmid # 45237 ; http://n2t.net/addgene:45237 ; RRID:Addgene_45237)

  • For your References section:

    Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. Wang E, Ballister ER, Lampson MA. J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. Epub 2011 Aug 15. 10.1083/jcb.201103044 PubMed 21844210
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