Cenp-B DBD INCENP TSS/AAA mCherry
(Plasmid
#45234)
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PurposeTarget INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Modifications to backbone
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
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Alt namepXW022
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SpeciesH. sapiens (human)
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Insert Size (bp)3895
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MutationINCENP C-terminal TSS mutated to AAA
- Promoter t7
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Tags
/ Fusion Proteins
- mCherry (C terminal on insert)
- CENP-B DBD, DNA binding domain of CENP-B
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer t7
- 3′ sequencing primer mCherry-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cenp-B DBD INCENP TSS/AAA mCherry was a gift from Michael Lampson (Addgene plasmid # 45234 ; http://n2t.net/addgene:45234 ; RRID:Addgene_45234) -
For your References section:
Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. Wang E, Ballister ER, Lampson MA. J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. Epub 2011 Aug 15. 10.1083/jcb.201103044 PubMed 21844210
