nonphosphorylatable Mis12-targeted Aurora B FRET sensor
(Plasmid
#45229)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerinvitrogen
- Total vector size (bp) 7984
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenon phosphorylatable Mis12-targeted Aurora B FRET sensor
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Alt namepDL007
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MutationT-A mutation in substrate sequence
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
nonphosphorylatable Mis12-targeted Aurora B FRET sensor was a gift from Michael Lampson (Addgene plasmid # 45229 ; http://n2t.net/addgene:45229 ; RRID:Addgene_45229) -
For your References section:
Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates. Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM. Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. Epub 2009 Jan 15. 10.1126/science.1167000 PubMed 19150808