Mis12 INCENP Δcen mCherry
(Plasmid
#45228)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMis12 INCENP Δcen mCherry
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Alt namepKL0022
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MutationCEN box deleted from INCENP (aa1-46)
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Tag
/ Fusion Protein
- VSVG (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mis12 INCENP Δcen mCherry was a gift from Michael Lampson & Susanne Lens (Addgene plasmid # 45228 ; http://n2t.net/addgene:45228 ; RRID:Addgene_45228) -
For your References section:
Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates. Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM. Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. Epub 2009 Jan 15. 10.1126/science.1167000 PubMed 19150808