mCherry KNL1 wt hardened and RVSF->AAAA
(Plasmid
#45226)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneunknown
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA
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Alt nameCASC5
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Alt namepDL020
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SpeciesH. sapiens (human)
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MutationRVSF 58 AAAA
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Entrez GeneKNL1 (a.k.a. AF15Q14, CASC5, CT29, D40, MCPH4, PPP1R55, Spc7, hKNL-1, hSpc105)
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Tag
/ Fusion Protein
- TEV S15 (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry KNL1 wt hardened and RVSF->AAAA was a gift from Iain Cheeseman & Michael Lampson (Addgene plasmid # 45226 ; http://n2t.net/addgene:45226 ; RRID:Addgene_45226) -
For your References section:
Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase. Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, Lampson MA. J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. Epub 2010 Mar 15. 10.1083/jcb.201001006 PubMed 20231380